|
KMID : 0352720120360030322
|
|
Journal of Ginseng Research
2012 Volume.36 No. 3 p.322 ~ p.326
|
|
|
Characterization of the Variability of Nucleoli in the Cells of Panax ginseng Meyer In Vivo and In Vitro
|
|
Yuliya A. Khrolenko
Olga L. Burundukova
Lyudmila S. Lauve
Tamara I. Muzarok
Vyacheslav V. Makhan’kov
Yuri N. Zhuravlev
|
|
|
Abstract
|
|
|
|
Results of karyological study of intact plants and some callus lines of Panax ginseng are presented. In the native plants of P. ginseng the nucleus with 1 nucleolus (90%) dominate, and nucleus with 2 nucleoli is rare. One nucleolar nucleus also dominate in interphase nuclei of cells of cultivated P. ginseng (from 2006), but we also found nucleus with 2 to 3 nucleoli in the same cell lines. Interphase nuclei of P. ginseng in long cultivated lines (from 1988) contain 1 to 9 nucleoli, with a predominance of nuclei containing from 3 to 4 nucleoli. It was shown that long-time cells (cultivated since 1988) had cytogenetic changes such as increase level of polyploid and aneuploid cells, increase of nucleoli number into interphase nucleus and decrease of nuclei/nucleoli ratio. These long-time cultivated cells had very low ginsenoside content.
|
|
|
KEYWORD
|
|
|
Panax ginseng, In vivo, In vitro, Nucleoli, Ginsenoside content,
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|